Detection of aneuploidy with digital polymerase chain reaction.

Detection of Aneuploidy with Digital PCR

The widespread use of genetic testing in high risk pregnancies has created strong interest in rapid and accurate molecular diagnostics for common chromosomal aneuploidies. We show here that digital polymerase chain reaction (dPCR) can be used for accurate measurement of trisomy 21 (Down’s Syndrome), the most common human aneuploidy. dPCR is generally applicable to any aneuploidy, does not depen...

Microfluidic digital PCR enables rapid prenatal diagnosis of fetal aneuploidy.

OBJECTIVE The purpose of this study was to demonstrate that digital polymerase chain reaction (PCR) enables rapid, allele independent molecular detection of fetal aneuploidy. STUDY DESIGN Twenty-four amniocentesis and 16 chorionic villus samples were used for microfluidic digital PCR analysis. Three thousand and sixty PCR reactions were performed for each of the target chromosomes (X, Y, 13, ...

Detection of Babesia ovis Using Polymerase Chain Reaction.

Application of digital polymerase chain reaction technology for noninvasive prenatal test

the chromosomal abnormalities, it was reported that Down syndrome (trisomy 21) occurs in 1 in 800 [3,4], Edward syndrome (trisomy 18) in 1 in 6,000 [5], and Patau syndrome (trisomy 13) in 1 in 10,000 fetal subjects [6]. Moreover, birth defects from Down syndrome comprise about 26% of all congenital birth defects [7,8]. As maternal age increases, fetal chromosomal aneuploidy becomes more prevale...

Detection of Plasmodium falciparum Directly from Blood Samples Using the Polymerase Chain Reaction